Point-of-care Blood Coagulation Monitoring Using Lateral Flow Device
نویسندگان
چکیده
A simple and affordable method of evaluating blood coagulation ability using microfluidic paperbased lateral flow assay (LFA) device is reported. Rabbit whole blood treated with citrate as anticoagulant was used in experiments. Re-establishment of blood coagulation cascade and creation of different coagulation abilities were achieved by adding CaCl2 solutions of varying concentrations. Since viscosity change of blood depends on its coagulation ability, travel distance of red blood cells (RBC) on nitrocellulose membrane can be used as an indicator of blood clotting time. RBC travel distance is found to decrease linearly with increasing CaCl2 concentration.
منابع مشابه
Blood coagulation screening using a paper-based microfluidic lateral flow device.
A simple approach to the evaluation of blood coagulation using a microfluidic paper-based lateral flow assay (LFA) device for point-of-care (POC) and self-monitoring screening is reported. The device utilizes whole blood, without the need for prior separation of plasma from red blood cells (RBC). Experiments were performed using animal (rabbit) blood treated with trisodium citrate to prevent co...
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